Journal: American Journal of Physiology - Cell Physiology

Article Title: MiR-4674 regulates angiogenesis in tissue injury by targeting p38K signaling in endothelial cells

doi: 10.1152/ajpcell.00542.2019

Figure Lengend Snippet: Interleukin 1 receptor-associated kinase 1 (IRAK1) and BICD cargo adaptor 2 (BICD2) are bona fide targets of miR-4674 in endothelial cells (ECs). A: discovery and validation of miR-4674 target genes. Human umbilical vein endothelial cells (HUVECs) transfected with miR-negative control (NSm) and miR-4674 mimics (miR-4674m) were subjected to microarray gene profiling. Potential gene targets were further narrowed down by sequential use of bioinformatics and prediction algorithms, RT-quantitative PCR, Western blot analyses, 3′-untranslated region (UTR) reporter studies, and microribonucleoprotein immunoprecipitation (miRNP-IP) analysis. B and C: HUVECs transfected with NSm or miR-4674m were subjected to RT-qPCR for IRAK1 and BICD2 expression (B) or Western blot analyses using antibodies to IRAK1, BICD2, and GAPDH (n = 3 experiments) (C). D: luciferase activity of IRAK1 3′-untranslated region (UTR) and BICD2 3′-UTR normalized to total protein was quantified in HUVECs transfected with NSm, miR-4674m, NSi, or miR-4674i (n = 3 experiments). E: miRNP-IP analysis of enrichment of IRAK1 and BICD2 mRNA in HUVECs transfected with NSm or miR-4674m. *P < 0.01. RT-qPCR was performed to detect IRAK1, BICD2, or SMAD1. Results are representative of n = 3 replicates per group and 2 independent experiments. *P < 0.01. All data represent means ± SE.

Article Snippet: For DNA microarray gene chip analysis, HUVECs were transfected with 30 nM miRNA negative control or miR-4674 mimics for 24 h. Cells were collected into RNeasy mini kit (Qiagen) and sent for two-color, 4 × 44 K format (Agilent Technologies) human whole genome oligo microarray service (ArrayStar).

Techniques: Transfection, Negative Control, Microarray, Real-time Polymerase Chain Reaction, Western Blot, Immunoprecipitation, Quantitative RT-PCR, Expressing, Luciferase, Activity Assay

Journal: PLoS ONE

Article Title: Di (2-ethylhexyl) Phthalate Exposure Impairs Growth of Antral Follicle in Mice

doi: 10.1371/journal.pone.0148350

Figure Lengend Snippet: (A) apoptotic processes and (B) negative regulation of cell proliferation related genes expressions, via microarray, in control and DEHP-treatment groups.

Article Snippet: We used 6 μg of high quality RNA labeled with Cy5, and hybridized to a mouse oligo microarray (Phalanx Mouse Whole Genome One Array TM ; Phalanx Biotech Group, Palo Alto, CA, USA).

Techniques: Microarray, Control

Journal: PLoS ONE

Article Title: Di (2-ethylhexyl) Phthalate Exposure Impairs Growth of Antral Follicle in Mice

doi: 10.1371/journal.pone.0148350

Figure Lengend Snippet: The green bars represent gene expression quantities of control and DEHP-treatment groups microarray data, and the red bars represent qPCR data in DEHP-treatment groups compared with the control group. Compared to the control group, relative fold changes were presented as mean ± SD. All experiments were repeated at least three times independently. (* P < 0.05; ** P < 0.01).

Article Snippet: We used 6 μg of high quality RNA labeled with Cy5, and hybridized to a mouse oligo microarray (Phalanx Mouse Whole Genome One Array TM ; Phalanx Biotech Group, Palo Alto, CA, USA).

Techniques: Gene Expression, Control, Microarray

Journal: Clinical Case Reports

Article Title: Prenatal diagnosis of complex rearrangement of chromosome 21: The significance of interphase and metaphase fluorescence in situ hybridization and comparative genomic hybridization

doi: 10.1002/ccr3.22

Figure Lengend Snippet: Microarray characterization of a complex rearrangement of chromosome 21. Microarray plot showing (1) a two-copy gain of 25 oligonucleotide probes from the long arm of chromosome 21 at 21q11.2, ˜220 kb in size; immediately followed by (2) a single-copy gain of 285 oligonucleotide probes from 21q11.2-21q21.1, ˜2.74 Mb in size; immediately followed by (3) a two-copy gain of 85 oligonucleotide probes from 21q21.1, ˜860 kb in size. Also present are (4) a single-copy gain of 107 oligonucleotide probes from 21q21.1, ˜1.35 Mb in size; (5) a single-copy loss of 153 oligonucleotide probes from 21q21.3q-21q22.11, ˜3.77 Mb in size; (6) a single-copy gain of 483 oligonucleotide probes from 21q22.12.-21q22.2, ˜5.21 Mb in size; and (7) a single-copy gain of 528 oligonucleotide probes from terminal 21q22.2-21q22.3, ˜5.89 Mb in size. Probes are ordered on the x -axis according to physical mapping positions, with the most proximal q-arm probes to the left and the most distal q-arm probes to the right. Values along the y -axis represent log2 ratios of patient:control signal intensities. Results are visualized using Genoglyphix® (Signature Genomics, Spokane WA).

Article Snippet: The analysis of aCGH of the umbilical cord specimen, using a 135K feature whole-genome microarray (SignatureChip® Oligo SolutionTM version 2.0, custom designed by Signature Genomic Laboratories, LLC, made by Roche NimbleGen®, Madison, WI) identified a complex rearrangement of 21q; six gains including DSCR and one loss, resulting in both partial trisomy 21q and partial monosomy 21q (Fig. ).

Techniques: Microarray, Control